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Custom Digital PCR (dPCR) assays for DNA methylation refer to the development and utilization of specialized digital PCR techniques to analyze and quantify DNA methylation patterns at specific loci or regions of interest in a genome. DNA methylation is an epigenetic modification that involves the addition of methyl groups to cytosine residues, often occurring at CpG dinucleotides.
In the context of custom dPCR assays for DNA methylation, researchers design and customize PCR assays to target specific CpG sites or regions where DNA methylation is of interest. Digital PCR is a sensitive and precise method that allows for the absolute quantification of DNA molecules, enabling the accurate measurement of methylation levels at targeted loci.
Customization in this context may involve the design of primers and probes specific to the regions of interest, and the development of assays tailored to the study of particular genes or genomic regions. These assays are particularly valuable in epigenetic research, as they provide a high level of resolution and sensitivity in detecting and quantifying DNA methylation events, which can be crucial for understanding gene regulation, disease mechanisms, and potential therapeutic targets.
