Digital polymerase chain reaction (dPCR) is a biotechnological refinement of conventional polymerase chain reaction methods that can be used to directly quantify and clonally amplify nucleic acids strands including DNA, cDNA, or RNA. The key difference between dPCR and traditional PCR lies in the method of measuring nucleic acids amounts, with the former being a more precise method than PCR. Like PCR, in dPCR, a single reaction is also performed within a sample, but the sample is divided into numerous partitions, with the reaction being performed in each partition separately. With this division, nucleic acid quantities may be collected and measured more accurately and sensitively. The technique is regularly used for clonal amplification of materials for next-generation sequencing and has shown beneficial for analysing changes in gene sequences, including copy number variants and point mutations. Droplet Digital PCR (ddPCR) is a technique for dPCR in which a 20-microliter sample reaction is split into 20,000 nanoliter-sized oil droplets using a water-oil emulsion technique, thermocycled in a microfluidic cartridge, and the fluorescence amplitude of each droplet in each sample well is read using a droplet scanner.