All-in-one Digital PCR System RS32 SYSTEM
1. Fully automatic
Combined the PCR Amplifying Apparatus and the Biochip Scanner within a singular, advanced device.
2. High-Throughput
3. Multi-Color Fluorescence Channels
4. Open platform
Compatible with third-party reagents, providing the system with greater flexibility, suitable for broad application.
Digital PCR Application Solutions
Technical Specifications
Product Name
RS32 Fully Automatic All-in-One Digital PCR System
Model
RS3204
RS3205
RS3206
RS3207
Patent Technology
Mono FlexTM Droplet Generation Technology, Universal MacroTM Microfluidic Flat Heating Technology
Sample Types
All types of nucleic acids
Sample Throughput
4-32 samples/run
Cartridge Specifications
4 samples/cartridge, 8 cartridges/run
Dimension
500 (W) *750 (D) *600 (H) (mm)
Weight
≤ 50 kg
Sample Preparation
Manual loading of samples and droplet generating oils without additional manual operations
Droplet Generation
2 minutes
Droplet Count
20,000 - 25,000
PCR Amplification
Fully automatic integrated droplet generation and amplification system, supports PCR temperature gradient function
Reagent Type
Compatible with various dye- and probe-based assays
Fluorescence Channels
4 colors
5 colors
6 colors
7 colors
Supported Dyes
FAM/HEX/ ROX/Cy5
FAM/HEX/ROX/Cy5/Cy5.5
Atto425/FAM/HEX/ ROX/
CY5/Cy5.5 or FAM/HEX/ROX/CY5/
Cy5.5/CY7
Atto425/FAM/HEX/ROX/ CY5/Cy5.5/CY7
Detection Method
CMOS, able to identify negative droplets without the need for reference dye
Sensitivity
Detectable mutation frequency as low as 0.001%, enabling single-copy detection
Dynamic Range
5 log
Precision
± 10%
Sample Recovery
Supports sample recovery by applying pressure to the cartridge
Cartridge Re-scanning
Supports long-term storage and re-scanning of cartridge
Key Features
Droplets formation
20,000 monodisperse droplets were generated in less than 2 minutes, providing partitioned units for Poisson distribution.
Easy to use
Simple workflow similar to qPCR experiments
Data quality control
Software can identify invalid droplet casting confidence in data quality
Integrated System
Small footprint and easy to move around
Flexibility
1-4 samples/cartridge, 1-32 samples/run
Multiplexing
4-7 fluorescence channels
Digital PCR Consumble
DPCR consumables are essential from sample preparation to thermal cycling and analysis. These consumables are crucial for maintaining the integrity of PCR experiments, preventing contamination, and ensuring precise sample handling and analysis.
Digital PCR Cartridge
The cartridge serves as a reaction container for partitioning PCR reaction mixture into 20,000 individual droplets. Each cartridge has 8 reaction chambers and can process up to 4 samples at a time. Each sample includes 2 reaction chambers.
Digital PCR Cartridge Sealing Cover
Digital PCR sealing covers are used to seal the sample loading wells, oil wells, and waste wells at each end of the Digital PCR cartridge. The sealing covers create an airtight space that ensures pressure in the PCR reaction and prevents aerosol leakage.
Heat Transfer Film
Heat transfer film ensure efficient and uniform heat transfer between the digital PCR cartridge and the heat plate of the PCR Amplifying Apparatus.
Droplet Generation Oil
Droplet generation Oil interacts with digital PCR mastermix to form water-in-oil droplets
Digital PCR Mastermix
The Digital PCR Master Mix contains the PCR enzyme, dNTP, buffer, and other components for digital PCR reactions. It interacts with droplet generating oils to form 20,000 individual droplets in which DNA or RNA templates go through independent PCR amplification.
FAQs
- Q: Do the instruments require regular calibration?A:
RainSure dPCR instruments are calibration-free if the operating procedures, as well as daily, monthly, and yearly maintenance schedules are followed. Understand that some laboratories require yearly calibration for audit purpose, such service is available upon request and report will be provided.
- Q: What do I do with empty (no sample) well(s)?A:
You can load a maximum of four samples on a single chip, well(s) in the absence of sample need(s) to be filled with oil and water.
- Q: How should the positive and negative droplets be classified when the fluorescence intensity of the scattergram is low? What are the specific standards?A: It is recommended to set up a control experiment to determine the threshold. A general fluorescence intensity threshold of 3000 can be used, alternatively the negative cluster could be used as a guide to differentiate the positive.
- Q: Can the software generate an experimental analysis report?A:
Yes, RainSure GeneCount Analysis software allows you to produce a report using the standard template and it can be exported in PDF.
- Q: What are the potential factors that lead to deviations in droplets generated?A:
1. The chip is left for too long (> 10 minutes) after the addition of droplet generation oil
2. Introduction of bubbles during sample addition
3. Pipetting accuracy
4. Not adding oil but mastermix first
Our Customers
Installation and Operation of the RainSure RS32 All-in-One Digital PCR System
Keep In Touch With Us
CONTACT US
+86-512-6750-3398
overseas-sales@rainsurebio.com
+86-15189171627
