Digital PCR Mastermix

Digital PCR master mix is a specialized solution containing all the necessary components for the dPCR reaction. It is specifically designed to enable accurate and precise amplification of target DNA or RNA molecules in the dPCR process.

The digital PCR master mix typically includes Taq DNA Polymerase, dNTPs, PCR buffer, as well as stabilizers and enhancers. It is provided as a ready-to-use solution, simplifying the experimental setup and reducing the risk of pipetting errors. It is important to handle the master mix with care to avoid contamination and degradation of the components.

During the dPCR experiment, the master mix is mixed with the DNA or RNA sample and distributed into the individual reaction vessels. The PCR Amplifying Apparatus then thermally cycles the reaction mixture, repeatedly heating and cooling it to facilitate DNA amplification within each reaction volume.

The components and formulation of the digital PCR master mix are optimized to ensure accurate quantification and minimize the formation of false positives or negatives. The master mix's composition and quality play a significant role in achieving reliable and reproducible results in digital PCR assays.