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A laboratory-grade portable real-time fluorescence quantitative PCR system, part of the FAST-16 series, has been developed specifically for clinical diagnostic applications. It supports 3-6 fluorescence detection channels and can accommodate up to 16 samples in a single run. The system features an integrated touch screen interface and an intuitive software package, allowing for seamless experimental procedures and result analyses. Designed with a compact form factor and the option of an external battery, it ensures ease of transport and suitability for clinical settings. With its powerful functionality, the system enables precise relative quantification, melting curve analysis, and positive/negative sample analysis, delivering high sensitivity in clinical diagnostics. The built-in 7" HD capacitive screen PDA provides a user-friendly touch screen operation, simplifying experimental workflows. The system's 16x0.2ml reaction module is compatible with both eight-row tubes and single tubes, providing flexibility in sample handling. The optional external battery guarantees uninterrupted experiments, maintaining optimal performance in clinical laboratory environments.