1. DNA samples:
OD260/280 = 1.6-1.8
Concentration ≥ 10 ng/μL
Volume ≥ 10 μL
Shipped on ice (ice packs)
2. RNA samples:
OD260/280 = 1.8-2.0
Concentration ≥ 20 ng/μL
Volume ≥ 20 μL
Shipped on ice (dry ice)
3. ddPCR primers:
Concentration = 450 nM-900 nM
4. ddPCR probe:
Concentration = 250 nM
1. Run the setup file in administrator mode
2. Set English as the default language
Extremely low sample concentration. For borderline positive, it is necessary to carry out in depth analysis in combination with negative/ positive quality control materials
The target strain(s) might have been mutated, low nucleic acid extraction efficiency.
For use of third-party reagents, it is necessary to optimize and verify with RainSure dPCR system. Some parameters include primers and probes concentration, enzyme compatibility with RainSure droplet generation oil, and thermal cycling profile.
This is largely related to experimental (handling) error. It is recommended to place the pipette vertically with the tip against the bottom of the sample well in the cartridge when adding the mastermix to avoid creating air bubbles in the sample well. If air bubbles appear in the sample well, large bubbles will be created when generating droplets, resulting in a reduced number of droplets.
Also, please remember to always add oil first and then add the mastermix to ensure successful droplet generation. Nevertheless, it is always important to examine the goods upon receipt as well as monitor your storage temperature to rule out compromised quality due to logistics and storage.
1. The chip is left for too long (> 10 minutes) after the addition of droplet generation oil
2. Introduction of bubbles during sample addition
3. Pipetting accuracy
4. Not adding oil but mastermix first
