Droplet-Based PCR for Absolute Quantification of Mutations

Droplet-based PCR for the absolute quantification of mutations is a technique used to precisely measure the abundance of specific genetic mutations in a DNA sample. This approach allows researchers to determine the exact number of mutant copies within a sample, offering valuable information in various applications, including cancer research and diagnostics.

Example:

Suppose you are studying a viral infection, and there is a critical point mutation associated with antiviral drug resistance. Your goal is to quantify the exact number of viral genome copies carrying this mutation in patient samples using droplet-based PCR. In this application, droplet-based PCR allows for the accurate determination of the drug-resistant mutation's abundance in the viral population. This information can be crucial for assessing the potential success of antiviral treatments, monitoring the emergence of drug resistance, and guiding therapeutic decisions in the context of viral infections.