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Droplet-based PCR is a powerful technique used for the absolute quantification of circulating tumor DNA (ctDNA) in biological samples. ctDNA refers to small fragments of DNA shed by tumors into the bloodstream, and its quantification can provide valuable information about cancer dynamics, treatment response, and minimal residual disease.
Droplet-based PCR for ctDNA quantification involves isolating and amplifying specific genomic regions associated with the tumor. By partitioning the PCR reaction into numerous droplets, each containing a single DNA template, this technique enables precise quantification of ctDNA in a sample.
Example:
Let's consider a scenario where researchers are studying ctDNA in the blood of cancer patients to monitor the progression of the disease and assess the effectiveness of treatment. This application of droplet-based PCR allows researchers and clinicians to non-invasively monitor cancer progression, detect early signs of recurrence, and tailor treatment strategies based on the quantitative assessment of ctDNA in the bloodstream.
