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A For the use of reagents and equipment from different manufacturers, it is necessary to optimize and verify the entire detection system.
①Amplification specificity factors: the specificity of primers and probes, the ratio of components in the reagents, the amplification cycle conditions, etc.
② Selection of reagents: If you choose a brand of reagents with better quality, the probe method is preferred, the group is FAM, and the dye method is EVA-Green, etc.
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A If the quality control result is normal, it may be caused by a large number of non-specific amplification. The reasons may include: insufficient primer and probe specificity, poor composition ratio in the reagent, improper amplification conditions, etc.
It is not easy for the commercialized kits of RainSure to appear rainy, because the entire detection system is optimized and verified by the manufacturer through a lot of tests, and the use of other brand reagents with our company's reagents also requires certain optimization and exploration . optimal reaction conditions. It should be noted that a few rain-like droplets have little effect on the experimental results.
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A Try to restart , if it reappears, there may be improper operation, please contact our technical staff.
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A In addition to the problem of test operation, check the storage temperature and quality documents of the reagents. If there is a problem with the quality of the reagents due to logistics reasons, or the transportation time is too long (or improper storage), you can contact us to communicate related matters.
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A RainSure uses a microfluidic chip to form water-in-oil droplets, and there will be no empty reaction in the edge hole, so there is no need to calibrate the fluorescence channel.
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A At present , the inspection report of the fixed template can be exported , and the report customization function will be added in the future.
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A Yes, a chip can have up to four samples. Oil and water need to be added to the wells without samples. Even if there are less than 4 samples, one chip will be consumed.
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A RainSure digital PCR equipment has no crosstalk of fluorescence values between channels, no need for compensation
